Constructing DNA by polymerase recombination
نویسندگان
چکیده
منابع مشابه
Constructing DNA by polymerase recombination
Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to b...
متن کاملSPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
متن کاملDNA Recombination by XPCR
The first step of the Adleman-Lipton extract model in DNA computing is the combinatorial generation of libraries. In this paper a new method is proposed for generating a initial pool, it is a quaternary recombination of strings via application of null context splicing rules. Its implementation, based on a kind of PCR called XPCR, results to be convenient with respect to the standard methods, in...
متن کاملThe Role of DNA Polymerase 4 in Homologous Recombination
DNA double-strand breaks (DSBs), caused by either exogenous or endogenous factors, will lead to cell death unless repaired by DNA repair pathways that maintain genome stability. DNA polymerase 4 (Pol4) has been implicated in a pathway of DSB repair called microhomology-mediated endjoining (MMEJ), which uses short segments of microhomologies (5-25 bps) to repair DSBs. To determine the role of Po...
متن کاملConstructing Minimal Ancestral Recombination Graphs
By viewing the ancestral recombination graph as defining a sequence of trees, we show how possible evolutionary histories consistent with given data can be constructed using the minimum number of recombination events. In contrast to previously known methods, which yield only estimated lower bounds, our method of detecting recombination always gives the minimum number of recombination events if ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.13.3983